Nheyo yeOligo Synthesizer
Muminda yemamorekuru biology uye genetics research, kugona kugadzira DNA kunoita basa rakakosha.Kuumbwa kweDNA kunosanganisira kugadzirwa kwemamorekuru eDNA nekuronga ma nucleotides mune imwe hurongwa.Kuti uite izvi, masayendisiti anovimba nechombo chine simba chinozivikanwa se oligonucleotide synthesizer, inozivikanwawo seDNA synthesizer.
Oligonucleotide synthesizer chiridzwa chakaomarara chinongogadzira mapfupi eDNA mamorekuru anonzi oligonucleotides.Aya ma tambo mapfupi eDNA anowanzo kuita gumi kusvika ku100 nucleotide pakureba uye zvakakosha zvivharo mumhando dzakasiyana siyana dzekushandisa dzinosanganisira polymerase chain reaction (PCR), gene synthesis, genetic engineering, uye DNA kutevedzana.
Oligonucleotide synthesizers inoshanda pamusimboti wehunyanzvi unozivikanwa sesolid-phase synthesis.Nzira iyi yakatanga kuitwa nemuwani wemubairo weNobel Dr. Marvin Caruthers mumakore ekuma1970 uye yakakwenenzverwa nekufamba kwemakore kuti iwedzere kuumbwa kweDNA sequences.Oligonucleotide synthesis inoitwa nekuwedzera nhanho yekuwedzera nucleotide zvisaririra kune 5'-terminus yeketani inokura kusvika iyo inoteedzana inodiwa yaunganidzwa.Yese yekuwedzera inonzi inonzi synthesis cycle uye ine maitiro mana emakemikari:
Danho 1: De-blocking (detritylation)----------Nhanho 2: Kubatana----------Nhanho 3: Kuvhara------------Nhanho 4: Oxidation
Iyi nzira inodzokororwa kune imwe neimwe nucleotide kusvikira kutevedzana kunodiwa kwawanikwa.Kune akareba oligonucleotides, kutenderera uku kungangoda kudzokororwa kakawanda kuti iite kutevedzana kwese. Iko kugona kunyatso kudzora nhanho yega yega yesynthesis cycle yakakosha kune oligonucleotide synthesizer.Iwo mareagents anoshandiswa, akadai semanucleotides uye activator, anofanirwa kunge ari emhando yepamusoro kuti ave nechokwadi chechokwadi uye chinoshanda synthesis.Uye zvakare, ma synthesizer anoda yakakwirira-chaiyo tembiricha kutonga uye mamwe mamiriro ezvakatipoteredza kusimudzira anodiwa coupling maitiro uye kudzivirira zvisingadiwe maitiro.
Kana imwe oligonucleotide yanyatsogadzirwa, inowanzotsemurwa kubva patsigiro yakasimba uye inocheneswa kubvisa chero mapoka ekudzivirira asara kana tsvina.Iwo akacheneswa oligonucleotides anobva agadzirira kuzasi kwekushandisa.
Kufambira mberi mune tekinoroji kwakagonesa kuvandudzwa kwepamusoro-kuburikidza oligonucleotide synthesizer inokwanisa panguva imwe chete synthesizing mazana kana kunyange zviuru zve oligonucleotides.Zviridzwa izvi zvinoshandisa microarray-based synthesis tekinoroji, zvichiita kuti vaongorori vakurumidze kuburitsa maraibhurari mahombe eoligonucleotide kune zvakasiyana siyana zvekutsvaga.
Muchidimbu, misimboti kuseri kwe oligonucleotide synthesizer inotenderera kune yakasimba-chikamu synthesis matekiniki, ayo anosanganisira nhanho yekuwedzera yemanucleotides pane yakasimba rutsigiro.Kunyatso kudzora kweiyo synthesis kutenderera uye emhando yepamusoro reagents akakosha pakuita kwakaringana uye kunoshanda synthesis.Oligo synthesizer inoita basa rakakosha mukutsvagisa kweDNA, zvichiita kuti masayendisiti agadzire tsika oligonucleotides kune akasiyana maapplication, zvichibatsira mukufambira mberi mubiotechnology uye genetic research.
Nguva yekutumira: Aug-01-2023