Oligo yekuchenesa equipmnt yeOligo primer synthesis
Oligo yekuchenesa equipmnt yeOligo primer synthesis,
oligo synthesis rutsigiro muchina,
Tsanangudzo
| Mabhodhi anoenderana | 1, 3, 5, 8. |
| Sefa | blow filtration, kusveta kusefa |
| Nhamba yemajekiseni ports | 5, 6, 7, 8, 9, 10. |
| Inoenderana ndiro mhando | C18 ndiro, yakadzika tsime ndiro, synthetic ndiro (inoenderana neakawanda mahwendefa ekugadzira), microtiter plate |
| Module | axis imwe chete kana mbiri mbiri |
| Voltage | 220V |
| Warranty | 1 gore |
| Custom | Kugamuchirwa |
Nzira dzakasiyana dzekunatsa
1. Gel Electrophoresis Chromatography Kucheneswa
Shandisa denaturing polyacrylamide gel electrophoresis chromatography yekuchenesa.Iyo denaturing agent inowanzoita 4M formamide kana 7M urea, huwandu hweacrylamide huri pakati pe5-15%, uye chikamu chemethacrylamide chinonyanya pakati pe2-10%.
Mushure me electrophoresis, chinzvimbo cheiyo nucleic acid bhendi inoda kutariswa pasi pekuvhenekesa kwechiedza che ultraviolet, gel ine chinangwa chenyucleic acid inogurwa, iyo nucleic acid inopwanywa uye yakanyungudutswa, uyezve iyo leaching mhinduro inonyungudika, yakabviswa munyu. quantified uye lyophilized.
2. DMT-On, HPLC Kucheneswa
Sarudza iyo DMT-On modhi panguva yekubatanidza, iyo isina kuchena chigadzirwa yaive centrifuged uye yakanamirwa pakamuri tembiricha kubvisa yakawandisa ammonia mushure meaminolysis.
Kuparadzana kwakaitwa pachishandiswa C18 column ine acetonitrile uye 10% triethylamine-acetic acid (TEAA) seyeroent.Mushure mokunge kujekesa kwapera, kunosimbiswa, uye ipapo boka reDMT rinobviswa ne trifluoroacetic acid.Mushure mekusagadzikana, mamwe mamunyu uye mamorekuru madiki anobviswa kuburikidza nechubhu yakachekwa, uye pakupedzisira inobviswa.
Iyi nzira inogona kuwana chigadzirwa nekuchena kwepamusoro, asi zvakakosha kubhadhara kune kuitika kwekutsvaira.
3. DMT-Off, HPLC Kucheneswa
Sarudza iyo DMT-Off panguva synthesis, uye crude chigadzirwa akanga centrifuged uye akaiswa pakamuri tembiricha kubvisa yakawandisa ammonia pashure ammonolysis.
Kuparadzanisa kwakaitwa uchishandisa C18 column ine acetonitrile uye 10% triethylamine-acetic acid mumvura seyakajeka.Mushure mokunge kupatsanurwa kwapera uye kuverengwa, iyo aliquots inonzi lyophilized.
Iyi nzira inoda kunyatsogadziriswa mamiriro ekuparadzana, uye inogonawo kuwana mamorekuru akanyatsonangwa.
oligo synthesis accerry midziyo ye dna rna synthesis cycle, inokodzera yakajairwa primer, uye imwe mapfupi ketani primer, inoita iyo oligo yakanyanya kuchena.
china mugadziri we oligo synthesis midziyo ine yakanaka mhando uye yakanakisa sevhisi.
