Ose ari maviri DNA neRNA synthesis yakavakirwa pane yakasimba-chikamu synthesis zano uye phosphoramidite chemistry, DNA synthesizer inogona kushandiswa kugadzira RNA kana RNA analogues pasina kumwe kugadziridzwa, uye reagents muDNA synthesis inogona kushandiswa zvakananga muRNA uye artificial nucleic acids. ' synthesis.Sezvinoratidzwa muMufananidzo 1, iyo 2'-hydroxy muRNA phosphoramidite inodzivirirwa neboka rekudzivirira silyl, kureva t-butyldimethylsilyl (TBDMS), kudzivirira maitiro epadivi pane isina kusimba 2'-hydroxy boka.Boka rakakura reTBMS rakatadzisa kuita pakati pephosphoramidite ne5'-hydroxy boka parutsigiro rwakasimba, uye nguva yakareba yekubatanidza inodiwa kuti uwane kugutsa kushanda kwekubatanidza.
Zvakafanana neDNA synthesis, iyo RNA yakasanganiswa yakatanga kutsemurwa kubva pakutsigirwa kwakasimba neaminolysis, ipapo boka reTBDMS rakatsemurwa ne tetrabutylammonium fluoride (TBAF) kana trimethylamine trihydrofluoride.Crude RNA inogona kucheneswa nekudzokororwa kubva kudoro uye HPLC.
Mufananidzo 1. Chimiro chemakemikari yezvivakwa zvekuvaka muDNA uye RNA synthesis.
a) dABz phosphoramidite uye b) rABz 2'-OTBDMS phosphoramidite.
Kugadzirwa kweSiRNA mushonga kunoda chimiro analogues ekuzvarwa RNA kuwedzera biocompatibility, iyo 2'-hydroxy paRNA inogona kutsiviwa neMeO, F uye MOE boka, uye yakakiyiwa nucleic acid (LNA) inopawo kuita kwakanaka muRNA therapy. (Mufananidzo 2).Aya maphosphoramidites anopa maitiro akafanana seDNA type phosphoramidites mu synthesis, uye kushanda-up uye kucheneswa kweaya asiri echisikigo nucleic acids akafanana neakaberekerwa DNA.
Mufananidzo 2. Chimiro chemakemikari yezvivakwa zvekuvaka mu siRNA mishonga.a) dABz 2-MeO phosphoramidite;b) dABz 2-F phosphoramidite;c) dABz 2-MOE phosphoramidite uye d) dABz Yakakiyiwa phosphoramidite.
Phosphoramiditesakakosha pakuumbwa kwemajini, kusanganisira kunyanya mhuri dzeDNA neRNA uye zvinobva kwavari, ese phosphoramidite pamusoro apa tinogona kupa mupakeji.
Isu tiri kugara tichinatsiridza mashandiro ezvigadzirwa zvedu, tichikwenenzvera maitiro edu ekugadzira uye kuwana iwo mameseji chaiwo.Isu hatikupe chete zvigadzirwa zvemhando yepamusoro, asi zvakare tinokupa iwe kudzidziswa uye sevhisi.Isu takashongedzerwa nevashandi vehunyanzvi manejimendi uye yakanakisa tekinoroji timu uye vashandi vekugadzirisa kuti vape vatengi vedu yakakwana mushure mekutengesa sevhisi.
Nguva yekutumira: Aug-09-2022